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首页> 外文OA文献 >Isolasi, Identifikasi Dan Evaluasi Antagonisme Terhadap Fusarium Oxysporum F.sp. Cubense (Foc) Secara in Vitro Dari Jamur Endofit Tanaman Pisang [Isolation, Identification and Evaluation of Antagonism to Fusarium Oxysporum F.sp. Cubense (Foc) Under in Vitro Conditions From Endophytic Fungi of Musa SP.]
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Isolasi, Identifikasi Dan Evaluasi Antagonisme Terhadap Fusarium Oxysporum F.sp. Cubense (Foc) Secara in Vitro Dari Jamur Endofit Tanaman Pisang [Isolation, Identification and Evaluation of Antagonism to Fusarium Oxysporum F.sp. Cubense (Foc) Under in Vitro Conditions From Endophytic Fungi of Musa SP.]

机译：尖孢镰刀菌拮抗作用的分离，鉴定和评价。香蕉植物内生蘑菇的离体成虫（Foc）[对镰刀菌F.sp.sp.的拮抗作用的分离，鉴定和评价。穆萨SP的内生真菌在体外条件下的Cubense（Foc）。

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摘要

Isolation of endophytic fungi was done to find alternative microorganisms as antifungal agent against Fusarium oxysporum f.sp. cubense (Foc), causing Panama disease (fusarium wilt) on Musa spp. The research objectives were 1) to isolate and identify endophytic fungi colonize cavendish (AAA) and rejang (AA) bananas growing on PT Natural Tropical Fruit (NTF) Plantation in East Lampung; and 2) to evaluate for their antagonistic activity against Foc under in vitro conditions. The results indicated 46 isolates of endophytic fungi that were isolated from leaves, saplings and petioles of cavendish banana; and leaves and petioles of rejang banana. The isolated endophytic fungi belonging to the group of Colletotrichum, Fusarium, Guignardia, Penicillium, Talaromyces and non sporulating endophytic fungi that were classified as unidentified isolate. Fourteen isolates (30.43 %) of those 46 fungi isolates tested, showed varying degree of antagonism to Foc. The best three isolates that have strong antagonistic activities were Talaromyces sp. 27-4 (M), unidentified 23-3/11 (B) and unidentified 26-5/K (L). Fusarium oxysporum 22-3/F (I), Penicillium sp. 20-2/H (J), Talaromyces sp. 27-4 (M) and unidentified 21-1/6 (A) inhibited the growth of Foc by producing both volatile and unvolatile metabolites. The strongest inhibition (53.17 %) by volatile inhibitory subtance was produced by F. oxysporum 22-3/F (I), while the strongest inhibition (65.98 %)by unvolatile inhibitory subtance was produced by Penicillium sp. 20-2/H (J).
机译：进行内生真菌的分离以找到替代的微生物作为针对尖孢镰刀菌（Fusarium oxysporum f.sp.）的抗真菌剂。 cubense（Foc），在Musa spp上引起巴拿马病（枯萎病）。研究目的是：1）分离和鉴定东楠榜省PT天然热带水果（NTF）种植园上种植的内生真菌殖民地（AAA）和rejang（AA）香蕉； 2）评估其在体外条件下对Foc的拮抗活性。结果表明，从卡文迪纳香蕉的叶子，幼树和叶柄中分离出46种内生真菌。和rejang香蕉的叶子和叶柄。分离出的内生真菌属于炭疽菌，镰刀菌，圭亚那，青霉属，毛囊菌和非孢子化内生真菌，它们被分类为未鉴定的分离物。在这46种真菌中，有14种（占30.43％）对Foc表现出不同程度的拮抗作用。具有强烈拮抗活性的最好的三种分离株是Talaromyces sp。 27-4（M），未知23-3 / 11（B）和26-5 / K（L）。尖孢镰刀菌22-3 / F（I），Penicillium sp。 20-2 / H（J），Talaromyces sp.。 27-4（M）和未知的21-1 / 6（A）通过产生挥发性和非挥发性代谢物来抑制Foc的生长。尖孢镰刀菌22-3 / F（I）对挥发性抑制物质的抑制作用最强（53.17％），而青霉菌对非挥发性抑制物质的抑制作用最强（65.98％）。 20-2 /小时（J）。

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